Design and Application of Noncontinuously Binding Probes Used for Haplotyping and Genotyping
نویسندگان
چکیده
منابع مشابه
Design and application of noncontinuously binding probes used for haplotyping and genotyping.
BACKGROUND Many methods for genotyping use melting temperature (Tm) of sequence-specific probes. Usually the probes hybridize to a continuous stretch of DNA that contains the variant(s). In contrast, hybridization of noncontinuous probes to a template can form bulges. This report generates guidelines for the design of noncontinuous probes. METHODS We used software to predict hybridization str...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2008
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2007.100487